7kbf

H1.8 bound nucleosome isolated from metaphase chromosome in Xenopus egg extract (oligo fraction)H1.8 bound nucleosome isolated from metaphase chromosome in Xenopus egg extract (oligo fraction)

Structural highlights 7kbf is a 11 chain structure with sequence from Xenopus laevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4.42Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B4_XENLA Publication Abstract from PubMed Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we devise the template-, reference- and selection-free (TRSF) cryo-EM pipeline to simultaneously reconstruct cryo-EM structures of protein complexes from interphase or metaphase chromosomes. The reconstructed interphase and metaphase nucleosome structures are on average indistinguishable from canonical nucleosome structures, despite DNA sequence heterogeneity, cell-cycle-specific posttranslational modifications, and interacting proteins. Nucleosome structures determined by a decoy-classifying method and structure variability analyses reveal the nucleosome structural variations in linker DNA, histone tails, and nucleosome core particle configurations, suggesting that the opening of linker DNA, which is correlated with H2A C-terminal tail positioning, is suppressed in chromosomes. High-resolution (3.4-3.5 A) nucleosome structures indicate DNA-sequence-independent stabilization of superhelical locations +/-0-1 and +/-3.5-4.5. The linker histone H1.8 preferentially binds to metaphase chromatin, from which chromatosome cryo-EM structures with H1.8 at the on-dyad position are reconstituted. This study presents the structural characteristics of nucleosomes in chromosomes. Structural features of nucleosomes in interphase and metaphase chromosomes.,Arimura Y, Shih RM, Froom R, Funabiki H Mol Cell. 2021 Nov 4;81(21):4377-4397.e12. doi: 10.1016/j.molcel.2021.08.010. , Epub 2021 Sep 2. PMID:34478647 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Histone 3D structures

spinqualitylabelsall models 7kbf, resolution 4.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image