7fad

Crystal structure of Xenopus GCP2-N terminal domain and Mzt2Crystal structure of Xenopus GCP2-N terminal domain and Mzt2

Structural highlights 7fad is a 6 chain structure with sequence from Xenopus laevis and Xenopus tropicalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.204Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6DDJ4_XENLA Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.[RuleBase:RU363050] Publication Abstract from PubMed The primary cilium, a microtubule-based sensory organelle, undergoes cycles of assembly and disassembly that govern the cell cycle progression critical to cell proliferation and differentiation. Although cilia assembly has been studied extensively, the molecular mechanisms underlying cilia disassembly are less well understood. Here, we uncover a gamma-tubulin ring complex (gamma-TuRC)-dependent pathway that promotes cilia disassembly and thereby prevents cilia formation. We further demonstrate that Kif2A, a kinesin motor that bears microtubule-depolymerizing activity, is recruited to the cilium basal body in a gamma-TuRC-dependent manner. Our mechanistic analyses show that gamma-TuRC specifically recruits Kif2A via the GCP2 subunit and its binding partner Mzt2. Hence, despite the long-standing view that gamma-TuRC acts mainly as a microtubule template, we illustrate that its functional heterogeneity at the basal body facilitates both microtubule nucleation and Kif2A recruitment-mediated regulation of ciliogenesis, ensuring cell cycle progression. Alpha gamma-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly.,Shankar S, Hsu ZT, Ezquerra A, Li CC, Huang TL, Coyaud E, Viais R, Grauffel C, Raught B, Lim C, Luders J, Tsai SY, Hsia KC Cell Rep. 2022 Nov 15;41(7):111642. doi: 10.1016/j.celrep.2022.111642. PMID:36384111 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tubulin 3D Structures

spinqualitylabelsall models 7fad, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image