7f90

Crystal structure of SARS auxiliary protein in complex with human nuclear proteinCrystal structure of SARS auxiliary protein in complex with human nuclear protein

Structural highlights

7f90 is a 6 chain structure with sequence from Homo sapiens and Severe acute respiratory syndrome-related coronavirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.39Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NS6_SARS Disrupts bidirectional nucleocytoplasmic transport by interacting with host RAE1-NUP98 complex (PubMed:33849972). Disrupts cell nuclear import complex formation also by tethering karyopherin alpha 2 and karyopherin beta 1 to the membrane (PubMed:17596301). Retention of import factors at the ER/Golgi membrane leads to a loss of transport into the nucleus (PubMed:17596301). Thereby prevents STAT1 nuclear translocation in response to interferon signaling, thus blocking the expression of interferon stimulated genes (ISGs) that display multiple antiviral activities (PubMed:17596301).^[1] ^[2]

References

↑ Frieman M, Yount B, Heise M, Kopecky-Bromberg SA, Palese P, Baric RS. Severe acute respiratory syndrome coronavirus ORF6 antagonizes STAT1 function by sequestering nuclear import factors on the rough endoplasmic reticulum/Golgi membrane. J Virol. 2007 Sep;81(18):9812-24. doi: 10.1128/JVI.01012-07. Epub 2007 Jun 27. PMID:17596301 doi:http://dx.doi.org/10.1128/JVI.01012-07
↑ Addetia A, Lieberman NAP, Phung Q, Hsiang TY, Xie H, Roychoudhury P, Shrestha L, Loprieno MA, Huang ML, Gale M Jr, Jerome KR, Greninger AL. SARS-CoV-2 ORF6 Disrupts Bidirectional Nucleocytoplasmic Transport through Interactions with Rae1 and Nup98. mBio. 2021 Apr 13;12(2). pii: mBio.00065-21. doi: 10.1128/mBio.00065-21. PMID:33849972 doi:http://dx.doi.org/10.1128/mBio.00065-21

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Frieman M, Yount B, Heise M, Kopecky-Bromberg SA, Palese P, Baric RS. Severe acute respiratory syndrome coronavirus ORF6 antagonizes STAT1 function by sequestering nuclear import factors on the rough endoplasmic reticulum/Golgi membrane. J Virol. 2007 Sep;81(18):9812-24. doi: 10.1128/JVI.01012-07. Epub 2007 Jun 27. PMID:17596301 doi:http://dx.doi.org/10.1128/JVI.01012-07

[2] Addetia A, Lieberman NAP, Phung Q, Hsiang TY, Xie H, Roychoudhury P, Shrestha L, Loprieno MA, Huang ML, Gale M Jr, Jerome KR, Greninger AL. SARS-CoV-2 ORF6 Disrupts Bidirectional Nucleocytoplasmic Transport through Interactions with Rae1 and Nup98. mBio. 2021 Apr 13;12(2). pii: mBio.00065-21. doi: 10.1128/mBio.00065-21. PMID:33849972 doi:http://dx.doi.org/10.1128/mBio.00065-21

[1]

[2]

7f90

Crystal structure of SARS auxiliary protein in complex with human nuclear proteinCrystal structure of SARS auxiliary protein in complex with human nuclear protein

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

7f90

Crystal structure of SARS auxiliary protein in complex with human nuclear proteinCrystal structure of SARS auxiliary protein in complex with human nuclear protein

Structural highlights

Function

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Search