7f26
Crystal structure of lysozymeCrystal structure of lysozyme
Structural highlights
Publication Abstract from PubMedIn situ microplates are small in size, crystal cultivation and operation are difficult, and the efficiency of crystal screening is relatively low. To solve this problem, a novel combined crystallization plate was designed for high-throughput crystal cultivation and in situ data collection. A frame was used to hold 48 in situ microplates, and the in situ microplates were sealed on one side with an ultralow background-scattering Kapton film. An automatic liquid handler (Mosquito) was used to add a liquid drop to the in situ microplates in the frame, and CrystalClear HD tape was used to seal the frame. A sealed frame holding 48 microplates was developed as a novel combined crystallization plate and was used for crystal cultivation under different conditions and in situ data collection at the synchrotron beamline. Moreover, individual microplates can be separated from the combined crystal plate and then fixed on a magnetic base or loaded onto a UniPuck for in situ data collection. Automatic grid scanning was used to locate crystals. The efficiency of the combined crystallization plate for crystal screening was verified. This method avoids the manual manipulation of crystals during crystal screening and diffraction data collection; therefore, the combined crystallization plate is suitable for large-scale screening of microcrystals. Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline.,Liang M, Yu L, Wang Z, Zhou H, Zhang Y, Wang Q, He J Acta Crystallogr F Struct Biol Commun. 2021 Sep 1;77(Pt 9):319-327. doi:, 10.1107/S2053230X21008104. Epub 2021 Aug 24. PMID:34473109[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||