7dsp

Anthranilate phosphoribosyltransferase variant Ser121Ala from Saccharomyces cerevisiae with Mg boundAnthranilate phosphoribosyltransferase variant Ser121Ala from Saccharomyces cerevisiae with Mg bound

Structural highlights 7dsp is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TRPD_YEAST Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA), the second step in tryptophan biosynthesis. Publication Abstract from PubMed Anthranilate phosphoribosyltransferase (AnPRT) catalyzes the transfer of the phosphoribosyl group of 5'-phosphoribosyl-1'-pyrophosphate (PRPP) to anthranilate to form phosphoribosyl-anthranilate. Crystal structures of AnPRTs from bacteria and archaea have previously been determined; however, the structure of Saccharomyces cerevisiae AnPRT (ScAnPRT) still remains unsolved. Here, crystal structures of ScAnPRT in the apo form as well as in complex with its substrate PRPP and the substrate analogue 4-fluoroanthranilate (4FA) are presented. These structures demonstrate that ScAnPRT exhibits the conserved structural fold of type III phosphoribosyltransferase enzymes and shares the similar mode of substrate binding found across the AnPRT protein family. In addition, crystal structures of ScAnPRT mutants (ScAnPRT(Ser121Ala) and ScAnPRT(Gly141Asn)) were also determined. These structures suggested that the conserved residue Ser121 is critical for binding PRPP, while Gly141 is dispensable for binding 4FA. In summary, these structures improved the preliminary understanding of the substrate-binding mode of ScAnPRT and laid foundations for future research. Crystal structures of anthranilate phosphoribosyltransferase from Saccharomyces cerevisiae.,Wu X, Zhang M, Kuang Z, Yue J, Xue L, Zhu M, Zhu Z, Khan MH, Niu L Acta Crystallogr F Struct Biol Commun. 2021 Mar 1;77(Pt 3):61-69. doi:, 10.1107/S2053230X21001989. Epub 2021 Mar 3. PMID:33682790 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphoribosyltransferase 3D structures

spinqualitylabelsall models 7dsp, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image