Acinetobacter baumannii response regulator AdeR with disordered N terminusAcinetobacter baumannii response regulator AdeR with disordered N terminus

Structural highlights

7cci is a 2 chain structure with sequence from Acinetobacter baumannii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.65Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q6TA00_ACIBA

Publication Abstract from PubMed

Bacterial two-component regulatory systems are ubiquitous environment-sensing signal transducers involved in pathogenesis and antibiotic resistance. The Acinetobacter baumannii two-component regulatory system AdeRS is made up of a sensor histidine kinase AdeS and a cognate response regulator AdeR, which together reduce repression of the multidrug-resistant efflux pump AdeABC. Herein we demonstrate that an N-terminal intrinsically disordered tail in AdeR is important for the upregulation of adeABC expression, although it greatly increases the susceptibility of AdeR to proteasome-mediated degradation. We also show that AdeS assembles into a hexameric state that is necessary for its full histidine kinase activity, which appears to occur via cis autophosphorylation. Taken together, this study demonstrates new structural mechanisms through which two-component systems can transduce environmental signals to impact gene expression and enlightens new potential antimicrobial approach by targeting two-component regulatory systems.

Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.,Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y iScience. 2021 Apr 26;24(5):102476. doi: 10.1016/j.isci.2021.102476. eCollection , 2021 May 21. PMID:34113820[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y. Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS. iScience. 2021 Apr 26;24(5):102476. PMID:34113820 doi:10.1016/j.isci.2021.102476

7cci, resolution 1.65Å

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