Structural highlightsFunctionENCAP_BRELN Shell component of a type 1 encapsulin nanocompartment. Assembles into proteinaceous shells 23-24 nm in diameter with 2-2.5 nm thick walls. Endogenous cargo protein DyP (dye-decolorizing peroxidase) is targeted to the interior via its C-terminal extension; only 1 DyP hexamer is incorporated into each shell. Empty shells can be isolated in the absence of cargo (PubMed:19172747, PubMed:27355101, PubMed:29166561). Cargo encapsulation probably precedes assembly of the nanocompartment; may assemble or disassemble via dimers, subcomplexes with a distinct preference for even numbers of subunits are detected (Probable) (PubMed:27355101). Nanocompartments are stable against mechanical forces; loaded nanocompartments are less stable than empty ones (PubMed:27355101). Nanocompartments are stable between pH 5-10; they aggregate at pH 9-10 and start to disassemble at pH 11. They are stable in 1M NaCl, 1 M MgCl(2) and 1M CaCl(2), unstable in 20% DMSO (dimethylsulfoxide) and are stable in 20% but not 40% ethanol (PubMed:29166561).[1] [2] [3] [4]
References
- ↑ Sutter M, Boehringer D, Gutmann S, Gunther S, Prangishvili D, Loessner MJ, Stetter KO, Weber-Ban E, Ban N. Structural basis of enzyme encapsulation into a bacterial nanocompartment. Nat Struct Mol Biol. 2008 Sep;15(9):939-47. PMID:19172747
- ↑ Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469
- ↑ Putri RM, Allende-Ballestero C, Luque D, Klem R, Rousou KA, Liu A, Traulsen CH, Rurup WF, Koay MST, Castón JR, Cornelissen JJLM. Structural Characterization of Native and Modified Encapsulins as Nanoplatforms for in Vitro Catalysis and Cellular Uptake. ACS Nano. 2017 Dec 26;11(12):12796-12804. PMID:29166561 doi:10.1021/acsnano.7b07669
- ↑ Snijder J, Kononova O, Barbu IM, Uetrecht C, Rurup WF, Burnley RJ, Koay MS, Cornelissen JJ, Roos WH, Barsegov V, Wuite GJ, Heck AJ. Assembly and Mechanical Properties of the Cargo-Free and Cargo-Loaded Bacterial Nanocompartment Encapsulin. Biomacromolecules. 2016 Aug 8;17(8):2522-9. PMID:27355101 doi:10.1021/acs.biomac.6b00469
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