6nbt
CRISPR Complex Subunit Csm3 from Staphylococcus epidermidis RP62aCRISPR Complex Subunit Csm3 from Staphylococcus epidermidis RP62a
Structural highlights
FunctionPublication Abstract from PubMedClustered regularly interspaced short palindromic repeats (CRISPR) and their associated Cas proteins provide an immune-like response in many prokaryotes against extraneous nucleic acids. CRISPR-Cas systems are classified into different classes and types. Class 1 CRISPR-Cas systems form multi-protein effector complexes that includes a guide RNA (crRNA) used to identify the target for destruction. Here we present crystal structures of Staphylococcus epidermidis Type III-A CRISPR subunits Csm2 and Csm3 and a 5.2 A resolution single-particle cryo-electron microscopy (cryo-EM) reconstruction of an in vivo assembled effector subcomplex including the crRNA. The structures help to clarify the quaternary architecture of Type III-A effector complexes, and provide details on crRNA binding, target RNA binding and cleavage, and intermolecular interactions essential for effector complex assembly. The structures allow a better understanding of the organization of Type III-A CRISPR effector complexes as well as highlighting the overall similarities and differences with other Class 1 effector complexes. Structural organization of a Type III-A CRISPR effector subcomplex determined by X-ray crystallography and cryo-EM.,Dorsey BW, Huang L, Mondragon A Nucleic Acids Res. 2019 Feb 13. pii: 5310821. doi: 10.1093/nar/gkz079. PMID:30759237[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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