6kyw

S8-mSRK-S8-SP11 complexS8-mSRK-S8-SP11 complex

Structural highlights

6kyw is a 4 chain structure with sequence from Brassica rapa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6011274Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q39276_BRACM

Publication Abstract from PubMed

Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In Brassica, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (S receptor kinase) and pollen determinant SP11 (S-locus Protein 11, also named SCR) from the S-locus. Although the structure of the B. rapa S9-SRK ectodomain (eSRK) and S9-SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in Brassica SI by determining the S8-eSRK-S8-SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK-SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK-SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in Brassica SI.

Mechanism of self/nonself-discrimination in Brassica self-incompatibility.,Murase K, Moriwaki Y, Mori T, Liu X, Masaka C, Takada Y, Maesaki R, Mishima M, Fujii S, Hirano Y, Kawabe Z, Nagata K, Terada T, Suzuki G, Watanabe M, Shimizu K, Hakoshima T, Takayama S Nat Commun. 2020 Oct 1;11(1):4916. doi: 10.1038/s41467-020-18698-w. PMID:33004803^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murase K, Moriwaki Y, Mori T, Liu X, Masaka C, Takada Y, Maesaki R, Mishima M, Fujii S, Hirano Y, Kawabe Z, Nagata K, Terada T, Suzuki G, Watanabe M, Shimizu K, Hakoshima T, Takayama S. Mechanism of self/nonself-discrimination in Brassica self-incompatibility. Nat Commun. 2020 Oct 1;11(1):4916. PMID:33004803 doi:10.1038/s41467-020-18698-w

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OCA

[1] Murase K, Moriwaki Y, Mori T, Liu X, Masaka C, Takada Y, Maesaki R, Mishima M, Fujii S, Hirano Y, Kawabe Z, Nagata K, Terada T, Suzuki G, Watanabe M, Shimizu K, Hakoshima T, Takayama S. Mechanism of self/nonself-discrimination in Brassica self-incompatibility. Nat Commun. 2020 Oct 1;11(1):4916. PMID:33004803 doi:10.1038/s41467-020-18698-w

[1]