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Crystal structure of drosophila Numb PTB domain and Pon peptide complexCrystal structure of drosophila Numb PTB domain and Pon peptide complex
Structural highlights
FunctionNUMB_DROME Required in determination of cell fate during sensory organ formation in embryos. Functions in nuclei and seems to interact with nucleic acids.[1] Publication Abstract from PubMedUneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including Drosophila neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes. Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division.,Shan Z, Tu Y, Yang Y, Liu Z, Zeng M, Xu H, Long J, Zhang M, Cai Y, Wen W Nat Commun. 2018 Feb 21;9(1):737. doi: 10.1038/s41467-018-03077-3. PMID:29467404[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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