5td8

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Crystal structure of an Extended Dwarf Ndc80 ComplexCrystal structure of an Extended Dwarf Ndc80 Complex

Structural highlights

5td8 is a 5 chain structure with sequence from Saccharomyces cerevisiae S288C and Vicugna pacos. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 7.531Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDC80_YEAST Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.[1] [2] [3] [4]

Publication Abstract from PubMed

The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a approximately 600 A long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends. The junction connects two alpha-helical coiled coils through regions of four-chain and three-chain overlap. The complexity of its structure depends on interactions among conserved amino-acid residues, suggesting a binding site for additional cellular factor(s) not yet identified.

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.,Valverde R, Ingram J, Harrison SC Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Zheng L, Chen Y, Lee WH. Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol. 1999 Aug;19(8):5417-28. PMID:10409732
  2. ↑ Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451
  3. ↑ McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:http://dx.doi.org/10.1101/gad.1040903
  4. ↑ Wigge PA, Jensen ON, Holmes S, Soues S, Mann M, Kilmartin JV. Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. J Cell Biol. 1998 May 18;141(4):967-77. PMID:9585415
  5. ↑ Valverde R, Ingram J, Harrison SC. Conserved Tetramer Junction in the Kinetochore Ndc80 Complex. Cell Rep. 2016 Nov 15;17(8):1915-1922. doi: 10.1016/j.celrep.2016.10.065. PMID:27851957 doi:http://dx.doi.org/10.1016/j.celrep.2016.10.065

5td8, resolution 7.53Å

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