5nyw
Anbu (ancestral beta-subunit) from Yersinia bercovieriAnbu (ancestral beta-subunit) from Yersinia bercovieri
Structural highlights
FunctionPublication Abstract from PubMedAnbu (ancestral beta-subunit) is homologous to HslV and 20S proteasomes. Based on its phylogenetic distribution and sequence clustering, Anbu has been proposed as the "ancestral" form of proteasomes. Here, we report biochemical data, small-angle X-ray scattering (SAXS) results, negative stain electron microscopy (EM) micrographs, and a crystal structure of the Anbu particle from Yersinia bercovieri (YbAnbu). All data are consistent with YbAnbu forming defined 12-14 subunit multimers that differ in shape from both HslV and 20S proteasomes. The crystal structure reveals that YbAnbu subunits form tight dimers, held together in part by the Anbu specific C-terminal helices. These dimers ("protomers") further assemble into a low rise left-handed staircase. The lock-washer shape of YbAnbu is consistent with the presence of defined multimers, X-ray diffraction data in solution and negative stain electron microscopy images. The presented structure suggests a possible evolutionary pathway from helical filaments to highly symmetric or pseudosymmetric multimer structures. YbAnbu subunits have the Ntn-hydrolase fold, a putative S1 pocket and conserved candidate catalytic residues Thr1, Asp17 and Lys32(33). Nevertheless, we did not detect any YbAnbu peptidase or amidase activity. However, we could document orthophosphate production from ATP catalyzed by the ATP-grasp protein encoded in the Yersinia bercovieri Anbu operon. The Y. bercovieri Anbu crystal structure sheds light on the evolution of highly (pseudo)symmetric multimers.,Piasecka A, Czapinska H, Vielberg MT, Szczepanowski R, Kiefersauer R, Reed S, Groll M, Bochtler M J Mol Biol. 2017 Dec 16. pii: S0022-2836(17)30590-9. doi:, 10.1016/j.jmb.2017.11.016. PMID:29258816[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||