5neq

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The structure of the G. violaceus guanidine II riboswitch P1 stem-loop with aminoguanidineThe structure of the G. violaceus guanidine II riboswitch P1 stem-loop with aminoguanidine

Structural highlights

5neq is a 1 chain structure with sequence from Gloeobacter violaceus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.69Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

The guanidine-II (mini-ykkC) riboswitch is the smallest of the guanidine-responsive riboswitches, comprising two stem loops of similar sequence. We have solved high-resolution crystal structures of both stem loops for the riboswitch from Gloeobacter violaceus. The stem loops have a strong propensity to dimerize by intimate loop-loop interaction. The dimerization creates specific binding pockets for two guanidine molecules, explaining their cooperative binding. Within the binding pockets the ligands are hydrogen bonded to a guanine at O6 and N7, and to successive backbone phosphates. Additionally they are each stacked upon a guanine nucleobase. One side of the pocket has an opening to the solvent, slightly lowering the specificity of ligand binding, and structures with bound methylguanidine, aminoguanidine, and agmatine show how this is possible.

The Structure of the Guanidine-II Riboswitch.,Huang L, Wang J, Lilley DMJ Cell Chem Biol. 2017 Jun 22;24(6):695-702.e2. doi:, 10.1016/j.chembiol.2017.05.014. Epub 2017 May 18. PMID:28529131[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Huang L, Wang J, Lilley DMJ. The Structure of the Guanidine-II Riboswitch. Cell Chem Biol. 2017 Jun 22;24(6):695-702.e2. doi:, 10.1016/j.chembiol.2017.05.014. Epub 2017 May 18. PMID:28529131 doi:http://dx.doi.org/10.1016/j.chembiol.2017.05.014

5neq, resolution 1.69Å

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OCA