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X-ray structure of carboplatin-encapsulated horse spleen apoferritin (rotating anode data)X-ray structure of carboplatin-encapsulated horse spleen apoferritin (rotating anode data)
Structural highlights
FunctionFRIL_HORSE Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Publication Abstract from PubMedThe second-generation Pt anticancer agent carboplatin (CBDCA) was encapsulated within the apo horse spleen ferritin (AFt) nanocage, and the X-ray structure of the drug-loaded protein was refined at 1.49 A resolution. Two Pt binding sites, different from the one observed in the cisplatin-encapsulated AFt, were identified in Ft subunits by inspection of anomalous electron density maps at two wavelengths and difference Fourier electron density maps, which provide the necessary sensitivity to discriminate between Pt from CBDCA and Cd ions that are present in the crystallization conditions. Pt centers coordinate to the NE2 atom of His49 and to the NE2 atom of His132, both on the inner surface of the Ft nanocage. X-ray Structure of the Carboplatin-Loaded Apo-Ferritin Nanocage.,Pontillo N, Ferraro G, Helliwell JR, Amoresano A, Merlino A ACS Med Chem Lett. 2017 Feb 28;8(4):433-437. doi: 10.1021/acsmedchemlett.7b00025., eCollection 2017 Apr 13. PMID:28435532[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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