5mb4

Crystal Structure of the Psathyrella asperospora lectin PAL in complex with GlcNAcCrystal Structure of the Psathyrella asperospora lectin PAL in complex with GlcNAc

Structural highlights 5mb4 is a 1 chain structure with sequence from Psathyrella. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: , , , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A1U7Q1Z0_9AGAR Publication Abstract from PubMed A lectin with strong cytotoxic effect on human colon cancer HT29 and monkey kidney VERO cells was recently identified from the Australian indigenous mushroom Psathyrella asperospora and named PAL. We herein present its biochemical and structural analysis using a multidisciplinary approach. Glycan arrays revealed binding preference towards N-acetylglucosamine (GlcNAc) and, to a lesser extent, towards sialic acid (Neu5Ac). Submicromolar and millimolar affinity was measured by surface plasmon resonance for GlcNAc and NeuAc, respectively. The structure of PAL was resolved by X-ray crystallography, elucidating both the protein's amino acid sequence as well as the molecular basis rationalizing its binding specificity. Proteins 2017. (c) 2017 Wiley Periodicals, Inc. Biophysical characterization and structural determination of the potent cytotoxic Psathyrella asperospora lectin.,Ribeiro JP, Ali Abol Hassan M, Rouf R, Tiralongo E, May TW, Day CJ, Imberty A, Tiralongo J, Varrot A Proteins. 2017 Feb 7. doi: 10.1002/prot.25265. PMID:28168856 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 5mb4, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image