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Structure of the snake adenovirus 1 hexon-interlacing LH3 protein, nativeStructure of the snake adenovirus 1 hexon-interlacing LH3 protein, native
Structural highlights
FunctionLH3_ADES1 structural component of the virion that presumably stabilizes the groups of hexons but is dispensable for assembly {eco Publication Abstract from PubMedAlthough non-human adenoviruses (AdVs) might offer solutions to problems posed by human AdVs as therapeutic vectors, little is known about their basic biology. In particular, there are no structural studies on the complete virion of any AdV with a non-mammalian host. We combine mass spectrometry, cryo-electron microscopy, and protein crystallography to characterize the composition and structure of a snake AdV (SnAdV-1, Atadenovirus genus). SnAdV-1 particles contain the genus-specific proteins LH3, p32k, and LH2, a previously unrecognized structural component. Remarkably, the cementing protein LH3 has a trimeric beta helix fold typical of bacteriophage host attachment proteins. The organization of minor coat proteins differs from that in human AdVs, correlating with higher thermostability in SnAdV-1. These findings add a new piece to the intriguing puzzle of virus evolution, hint at the use of cell entry pathways different from those in human AdVs, and will help development of new, thermostable SnAdV-1-based vectors. Structure of a Reptilian Adenovirus Reveals a Phage Tailspike Fold Stabilizing a Vertebrate Virus Capsid.,Menendez-Conejero R, Nguyen TH, Singh AK, Condezo GN, Marschang RE, van Raaij MJ, San Martin C Structure. 2017 Sep 6. pii: S0969-2126(17)30260-5. doi:, 10.1016/j.str.2017.08.007. PMID:28943338[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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