5dtc

UBL StructureUBL Structure

Structural highlights 5dtc is a 2 chain structure with sequence from Saccharomyces cerevisiae YJM1133. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The synthesis of eukaryotic ribosomes is a complex, energetically demanding process requiring the aid of numerous non-ribosomal factors such as the PeBoW complex. The mammalian PeBoW complex, comprised of Pes1, Bop1, and WDR12, is essential for the processing of the 32S pre-ribosomal RNA. Previous work in Saccharomyces cerevisiae has shown that release of the homologous proteins in this complex (Nop7, Erb1, and Ytm1, respectively) from pre-ribosomal particles requires Rea1 (Midasin or Mdn1 in humans), a large dynein-like protein. Midasin contains a C-terminal metal ion-dependent adhesion site (MIDAS) domain that interacts with the N-terminal ubiquitin-like (UBL) domain of Ytm1/WDR12 as well as the UBL domain of Rsa4/Nle1 in a later step in the ribosome maturation pathway. Here we present the crystal structure of the UBL domain of the WDR12 homologue from S. cerevisiae at 1.7 A resolution and demonstrate that human Midasin binds to WDR12 as well as Nle1 through their respective UBL domains. Midasin contains a well-conserved extension region upstream of the MIDAS domain required for binding WDR12 and Nle1 and the interaction is dependent upon metal ion-coordination as removal of the metal or mutation of residues that coordinate the metal ion diminishes the interaction. Mammalian WDR12 displays prominent nucleolar localization that is dependent upon active rRNA transcription. Based upon these results we propose that release of the PeBoW complex and subsequent release of Nle1 by Midasin is a well-conserved step in the ribosome maturation pathway in both yeast and mammalian cells. The Crystal Structure of the Ubiquitin-Like Domain of Ribosome Assembly Factor Ytm1 and Characterization of its Interaction with the AAA-ATPase Midasin.,Romes EM, Sobhany M, Stanley RE J Biol Chem. 2015 Nov 24. pii: jbc.M115.693259. PMID:26601951 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome biogenesis protein 3D structures

spinqualitylabelsall models 5dtc, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image