4v8y

Cryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation ComplexCryo-EM reconstruction of the 80S-eIF5B-Met-itRNAMet Eukaryotic Translation Initiation Complex

4v8y, resolution 4.30Å Show:Asymmetric Unit Biological Assembly Structural highlights 4v8y is a 10 chain structure with sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entries 4byl, 4byn, 4byo, 4byp, 4byq, 4byr and 4bys. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS26A_YEAST Publication Abstract from PubMed The last step in eukaryotic translational initiation involves the joining of the large and small subunits of the ribosome, with initiator transfer RNA (Met-tRNA(i)(Met)) positioned over the start codon of messenger RNA in the P site. This step is catalyzed by initiation factor eIF5B. We used recent advances in cryo-electron microscopy (cryo-EM) to determine a structure of the eIF5B initiation complex to 6.6 angstrom resolution from <3% of the population, comprising just 5143 particles. The structure reveals conformational changes in eIF5B, initiator tRNA, and the ribosome that provide insights into the role of eIF5B in translational initiation. The relatively high resolution obtained from such a small fraction of a heterogeneous sample suggests a general approach for characterizing the structure of other dynamic or transient biological complexes. Molecular architecture of a eukaryotic translational initiation complex.,Fernandez IS, Bai XC, Hussain T, Kelley AC, Lorsch JR, Ramakrishnan V, Scheres SH Science. 2013 Nov 15;342(6160):1240585. doi: 10.1126/science.1240585. Epub 2013, Nov 7. PMID:24200810 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transfer RNA (tRNA) 3D sructureseceptor for activated protein kinase C 1