4v6p

Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes)Structural characterization of mRNA-tRNA translocation intermediates (class 4b of the six classes)

4v6p, resolution 13.50Å Show:Asymmetric Unit Biological Assembly Structural highlights 4v6p is a 10 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 3j0x and 3j0y. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RS2_ECOLI Publication Abstract from PubMed Cryo-EM analysis of a wild-type Escherichia coli pretranslocational sample has revealed the presence of previously unseen intermediate substates of the bacterial ribosome during the first phase of translocation, characterized by intermediate intersubunit rotations, L1 stalk positions, and tRNA configurations. Furthermore, we describe the domain rearrangements in quantitative terms, which has allowed us to characterize the processivity and coordination of the conformational reorganization of the ribosome, along with the associated changes in tRNA ribosome-binding configuration. The results are consistent with the view of the ribosome as a molecular machine employing Brownian motion to reach a functionally productive state via a series of substates with incremental changes in conformation. Structural characterization of mRNA-tRNA translocation intermediates.,Agirrezabala X, Liao HY, Schreiner E, Fu J, Ortiz-Meoz RF, Schulten K, Green R, Frank J Proc Natl Acad Sci U S A. 2012 Mar 30. PMID:22467828 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures Transfer RNA (tRNA)