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Hen egg-white lysozyme structure from a spent-beam experiment at LCLS: refocused beamHen egg-white lysozyme structure from a spent-beam experiment at LCLS: refocused beam
Structural highlights
FunctionLYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1] Publication Abstract from PubMedX-ray free-electron laser sources such as the Linac Coherent Light Source offer very exciting possibilities for unique research. However, beam time at such facilities is very limited and in high demand. This has led to significant efforts towards beam multiplexing of various forms. One such effort involves re-using the so-called spent beam that passes through the hole in an area detector after a weak interaction with a primary sample. This beam can be refocused into a secondary interaction region and used for a second, independent experiment operating in series. The beam profile of this refocused beam was characterized for a particular experimental geometry at the Coherent X-ray Imaging instrument at LCLS. A demonstration of this multiplexing capability was performed with two simultaneous serial femtosecond crystallography experiments, both yielding interpretable data of sufficient quality to produce electron density maps. Characterization and use of the spent beam for serial operation of LCLS.,Boutet S, Foucar L, Barends TR, Botha S, Doak RB, Koglin JE, Messerschmidt M, Nass K, Schlichting I, Seibert MM, Shoeman RL, Williams GJ J Synchrotron Radiat. 2015 May 1;22(Pt 3):634-43. doi: 10.1107/S1600577515004002., Epub 2015 Apr 11. PMID:25931079[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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