4mtk

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Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion SystemCrystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System

Structural highlights

4mtk is a 6 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.322Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VGR1A_PSEAE Part of the H1 type VI secretion system (H1-T6SS) specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:21325275, PubMed:24794869). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein 1/Hcp1 (PubMed:26894531). Allows the delivery of the Tse6 toxin to target cells where it exerts its toxicity (PubMed:30177742).[1] [2] [3] [4]

References

  1. Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jove D, Filloux A. Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 2011 Apr 8;286(14):12317-27. doi: 10.1074/jbc.M110.193045. Epub 2011 , Feb 16. PMID:21325275 doi:http://dx.doi.org/10.1074/jbc.M110.193045
  2. Hachani A, Allsopp LP, Oduko Y, Filloux A. The VgrG proteins are "a la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 2014 Jun 20;289(25):17872-84. doi: 10.1074/jbc.M114.563429. Epub , 2014 May 2. PMID:24794869 doi:http://dx.doi.org/10.1074/jbc.M114.563429
  3. Spinola-Amilibia M, Davo-Siguero I, Ruiz FM, Santillana E, Medrano FJ, Romero A. The structure of VgrG1 from Pseudomonas aeruginosa, the needle tip of the bacterial type VI secretion system. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):22-33. doi: , 10.1107/S2059798315021142. Epub 2016 Jan 1. PMID:26894531 doi:http://dx.doi.org/10.1107/S2059798315021142
  4. Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S. Mechanism of loading and translocation of type VI secretion system effector Tse6. Nat Microbiol. 2018 Sep 3. pii: 10.1038/s41564-018-0238-z. doi:, 10.1038/s41564-018-0238-z. PMID:30177742 doi:http://dx.doi.org/10.1038/s41564-018-0238-z

4mtk, resolution 3.32Å

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OCA