4lx6
X-ray crystal structure of the M6C" riboswitch aptamer bound to 2-aminopyrimido[4,5-d]pyrimidin-4(3H)-one (PPAO)X-ray crystal structure of the M6C" riboswitch aptamer bound to 2-aminopyrimido[4,5-d]pyrimidin-4(3H)-one (PPAO)
Structural highlights
Publication Abstract from PubMedLigand-dependent control of gene expression is essential for gene functional analysis, target validation, protein production and metabolic engineering. However, the expression tools currently available are difficult to transfer between species and exhibit limited mechanistic diversity. Here we demonstrate how the modular architecture of purine riboswitches can be exploited to develop orthogonal and chimeric switches that are transferable across diverse bacterial species, modulating either transcription or translation, to provide tuneable activation or repression of target gene expres-sion, in response to synthetic non-natural effector molecules. Our novel riboswitch-ligand pairings are shown to regulate physiologically important genes required for bacterial motility in Escherichia coli and cell morphology in Bacillus subtilis. These findings are relevant for future gene function studies and antimicrobial target validation, whilst providing new modular and orthogonal regulatory components for deployment in synthetic biology regimes. Modular Riboswitch Toolsets for Synthetic Genetic Control in Diverse Bacterial Species.,Robinson CJ, Vincent HA, Wu MC, Lowe PT, Dunstan MS, Leys D, Micklefield J J Am Chem Soc. 2014 Jun 27. PMID:24971878[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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