4jrt

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Crystal structure of an A-form RNA duplex containing three GU base pairsCrystal structure of an A-form RNA duplex containing three GU base pairs

Structural highlights

4jrt is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 A. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.

Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.,Kondo J, Dock-Bregeon AC, Willkomm DK, Hartmann RK, Westhof E Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):634-9. doi:, 10.1107/S1744309113013018. Epub 2013 May 24. PMID:23722840[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Kondo J, Dock-Bregeon AC, Willkomm DK, Hartmann RK, Westhof E. Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):634-9. doi:, 10.1107/S1744309113013018. Epub 2013 May 24. PMID:23722840 doi:10.1107/S1744309113013018

4jrt, resolution 2.60Å

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