4ixr
RT fs X-ray diffraction of Photosystem II, first illuminated stateRT fs X-ray diffraction of Photosystem II, first illuminated state
Structural highlights
FunctionPSBA1_THEVB Photosystem II (PSII) is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H(2)O, generating O(2) and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors.[HAMAP-Rule:MF_01379][1] [2] [3] Publication Abstract from PubMedIntense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of Photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn(4)CaO(5) cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S(1)) and the first illuminated state (S(2)) of PS II. Our simultaneous XRD/XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn(4)CaO(5) cluster, opening new directions for future dynamics studies. Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature.,Kern J, Alonso-Mori R, Tran R, Hattne J, Gildea RJ, Echols N, Glockner C, Hellmich J, Laksmono H, Sierra RG, Lassalle-Kaiser B, Koroidov S, Lampe A, Han G, Gul S, Difiore D, Milathianaki D, Fry AR, Miahnahri A, Schafer DW, Messerschmidt M, Seibert MM, Koglin JE, Sokaras D, Weng TC, Sellberg J, Latimer MJ, Grosse-Kunstleve RW, Zwart PH, White WE, Glatzel P, Adams PD, Bogan MJ, Williams GJ, Boutet S, Messinger J, Zouni A, Sauter NK, Yachandra VK, Bergmann U, Yano J Science. 2013 Feb 14. PMID:23413188[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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OCA- Large Structures
- Thermosynechococcus vestitus
- Adams PD
- Alonso-Mori R
- Bergmann U
- Bogan MJ
- Boutet S
- DiFiore D
- Echols N
- Fry AR
- Gildea RJ
- Glatzel P
- Gloeckner C
- Grosse-Kunstleve RW
- Gul S
- Han G
- Hattne J
- Hellmich J
- Kern J
- Koglin JE
- Koroidov S
- Laksmono H
- Lampe A
- Lassalle-Kaiser B
- Latimer MJ
- Messerschmidt M
- Messinger J
- Miahnahri A
- Milathianaki D
- Sauter NK
- Schafer DW
- Seibert MM
- Sellberg J
- Sierra RG
- Sokaras D
- Tran R
- Weng T-C
- White WE
- Williams GJ
- Yachandra VK
- Yano J
- Zouni A
- Zwart PH