4haa

Structure of Ribonuclease Binase Glu43Ala/Phe81Ala MutantStructure of Ribonuclease Binase Glu43Ala/Phe81Ala Mutant

Structural highlights 4haa is a 4 chain structure with sequence from Bacillus intermedius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RN_BACIN This is a purine-specific ribonuclease. Publication Abstract from PubMed Ribonuclease from Bacillus intermedius (binase) is a small basic protein with antitumour activity. The three-dimensional structure of the binase mutant form Glu43Ala/Phe81Ala was determined at 1.98 A resolution and its functional properties, such as the kinetic parameters characterizing the hydrolysis of polyinosinic acid and cytotoxicity towards Kasumi-1 cells, were investigated. In all crystal structures of binase studied previously the characteristic dimer is present, with the active site of one subunit being blocked owing to interactions within the dimer. In contrast to this, the new mutant form is not dimeric in the crystal. The catalytic efficiency of the mutant form is increased 1.7-fold and its cytotoxic properties are enhanced compared with the wild-type enzyme. Structure and functional studies of the ribonuclease binase Glu43Ala/Phe81Ala mutant.,Mitkevich VA, Schulga AA, Trofimov AA, Dorovatovskii PV, Goncharuk DA, Tkach EN, Makarov AA, Polyakov KM Acta Crystallogr D Biol Crystallogr. 2013 Jun;69(Pt 6):991-6. doi:, 10.1107/S0907444913004046. Epub 2013 May 11. PMID:23695243 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribonuclease 3D structures

spinqualitylabelsall models 4haa, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image