4c6y

Ancestral PNCA (last common ancestors of Gram-positive and Gram- negative bacteria) beta-lactamase class AAncestral PNCA (last common ancestors of Gram-positive and Gram- negative bacteria) beta-lactamase class A

Structural highlights 4c6y is a 2 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.799Å Ligands: , , , , , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Consensus-sequence engineering has generated protein variants with enhanced stability, and sometimes, with modulated biological function. Consensus mutations are often interpreted as the introduction of ancestral amino acid residues. However, the precise relationship between consensus engineering and ancestral protein resurrection is not fully understood. Here, we report the properties of proteins encoded by consensus sequences derived from a multiple sequence alignment of extant, class A beta-lactamases, as compared to the properties of ancient Precambrian beta-lactamases resurrected in the laboratory. These comparisons considered primary sequence, secondary and tertiary structure, as well as stability and catalysis against different antibiotics. Out of the three consensus variants generated, one could not be expressed and purified (likely due to misfolding and/or low stability) and only one displayed substantial stability having substrate promiscuity, although to a lower extent than ancient beta-lactamases. These results: i) highlight the phenotypic differences between consensus variants and laboratory resurrections of ancestral proteins; ii) question interpretations of consensus proteins as phenotypic proxies of ancestral proteins; and iii) support the notion that ancient proteins provide a robust approach towards the preparation of protein variants having large numbers of mutational changes while possessing unique biomolecular properties. (c) Proteins 2014;. (c) 2014 Wiley Periodicals, Inc. Phenotypic comparisons of consensus variants versus laboratory resurrections of Precambrian proteins.,Risso VA, Gavira JA, Gaucher EA, Sanchez-Ruiz JM Proteins. 2014 Apr 8. doi: 10.1002/prot.24575. PMID:24710963 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-lactamase 3D structures

spinqualitylabelsall models 4c6y, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image