4by6
Yeast Not1-Not2-Not5 complexYeast Not1-Not2-Not5 complex
Structural highlights
FunctionNOT1_YEAST Acts as component of the CCR4-NOT core complex, which in the nucleus seems to be a general transcription factor, and in the cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT protein subcomplex negatively regulates the basal and activated transcription of many genes. Preferentially affects TC-type TATA element-dependent transcription. Could directly or indirectly inhibit component(s) of the general transcription machinery.[1] Publication Abstract from PubMedThe Ccr4-Not complex is involved in several aspects of gene expression, including mRNA decay, translational repression and transcription. We determined the 2.8-A-resolution crystal structure of a 120-kDa core complex of the Saccharomyces cerevisiae Not module comprising the C-terminal arm of Not1, Not2 and Not5. Not1 is a HEAT-repeat scaffold. Not2 and Not5 have extended regions that wrap around Not1 and around their globular domains, the Not boxes. The Not boxes resemble Sm folds and interact with each other with a noncanonical dimerization surface. Disruption of the interactions within the ternary complex has severe effects on growth in vivo. The ternary complex forms a composite surface that binds poly(U) RNA in vitro, with a site at the Not5 Not box. The results suggest that the Not module forms a versatile platform for macromolecular interactions. Structure and RNA-binding properties of the Not1-Not2-Not5 module of the yeast Ccr4-Not complex.,Bhaskar V, Roudko V, Basquin J, Sharma K, Urlaub H, Seraphin B, Conti E Nat Struct Mol Biol. 2013 Oct 13. doi: 10.1038/nsmb.2686. PMID:24121231[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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