4a15

Crystal structure of an XPD DNA complexCrystal structure of an XPD DNA complex

Structural highlights 4a15 is a 2 chain structure with sequence from Thermoplasma acidophilum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: , , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function XPD_THEAC ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair (NER) of DNA (By similarity). Publication Abstract from PubMed The XPD protein is a vital subunit of the general transcription factor TFIIH which is not only involved in transcription but is also an essential component of the eukaryotic nucleotide excision DNA repair (NER) pathway. XPD is a superfamily-2 5'-3' helicase containing an iron-sulphur cluster. Its helicase activity is indispensable for NER and it plays a role in the damage verification process. Here, we report the first structure of XPD from Thermoplasma acidophilum (taXPD) in complex with a short DNA fragment, thus revealing the polarity of the translocated strand and providing insights into how the enzyme achieves its 5'-3' directionality. Accompanied by a detailed mutational and biochemical analysis of taXPD, we define the path of the translocated DNA strand through the protein and identify amino acids that are critical for protein function. Functional and structural studies of the nucleotide excision repair helicase XPD suggest a polarity for DNA translocation.,Kuper J, Wolski SC, Michels G, Kisker C EMBO J. 2011 Nov 11;31(2):494-502. doi: 10.1038/emboj.2011.374. PMID:22081108 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Helicase 3D structures

spinqualitylabelsall models 4a15, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image