3zfs

Cryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrateCryo-EM structure of the F420-reducing NiFe-hydrogenase from a methanogenic archaeon with bound substrate

3zfs, resolution 4.00Å Show:Asymmetric Unit Biological Assembly Structural highlights 3zfs is a 3 chain structure with sequence from Methanothermobacter marburgensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 4Å Ligands: Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D9PYF9_METTM Publication Abstract from PubMed Methanogenic archaea use a [NiFe]-hydrogenase, Frh, for oxidation/reduction of F, an important hydride carrier in the methanogenesis pathway from H and CO. Frh accounts for about 1% of the cytoplasmic protein and forms a huge complex consisting of FrhABG heterotrimers with each a [NiFe] center, four Fe-S clusters and an FAD. Here, we report the structure determined by near-atomic resolution cryo-EM of Frh with and without bound substrate F. The polypeptide chains of FrhB, for which there was no homolog, was traced de novo from the EM map. The 1.2-MDa complex contains 12 copies of the heterotrimer, which unexpectedly form a spherical protein shell with a hollow core. The cryo-EM map reveals strong electron density of the chains of metal clusters running parallel to the protein shell, and the F-binding site is located at the end of the chain near the outside of the spherical structure. http://dx.doi.org/10.7554/eLife.00218.001. De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy.,Mills DJ, Vitt S, Strauss M, Shima S, Vonck J Elife. 2013;2:e00218. doi: 10.7554/eLife.00218. Epub 2013 Mar 5. PMID:23483797 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.