3vw5

From Proteopedia
Jump to navigation Jump to search

Crystal structure of sugar epimerase from ruminal bacteriumCrystal structure of sugar epimerase from ruminal bacterium

Structural highlights

3vw5 is a 3 chain structure with sequence from Ruminococcus albus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CEEP_RUMAL Catalyzes the reversible epimerization of cellobiose to 4-O-beta-D-glucopyranosyl-D-mannose (Glc-Man). Can also epimerize cellotriose to Glc-Glc-Man, cellotetraose to Glc-Glc-Glc-Man, and lactose to epilactose.[HAMAP-Rule:MF_00929][1] [2]

Publication Abstract from PubMed

Enzymatic epimerization is an important modification for carbohydrates to acquire diverse functions attributable to their stereoisomers. Cellobiose 2-epimerase (CE) catalyzes interconversion between d-glucose and d-mannose residues at the reducing end of beta-1,4-linked oligosaccharides. Here, we solved the structure of Ruminococcus albus CE (RaCE). The structure of RaCE showed strong similarity to those of N-acetyl-D-glucosamine 2-epimerase and aldose-ketose isomerase YihS with a high degree of conservation of residues around the catalytic center, although sequence identity between them is low. Based on structural comparison, we found that His184 is required for RaCE activity as the third histidine added to two essential histidines in other sugar epimerases/isomerases. This finding was confirmed by mutagenesis, suggesting a new catalytic mechanism for CE involving three histidines.

Crystal structure of Ruminococcus albus cellobiose 2-epimerase: structural insights into epimerization of unmodified sugar.,Fujiwara T, Saburi W, Inoue S, Mori H, Matsui H, Tanaka I, Yao M FEBS Lett. 2013 Apr 2;587(7):840-6. doi: 10.1016/j.febslet.2013.02.007. Epub 2013, Feb 24. PMID:23462136[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Ito S, Hamada S, Yamaguchi K, Umene S, Ito H, Matsui H, Ozawa T, Taguchi H, Watanabe J, Wasaki J, Ito S. Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus. Biochem Biophys Res Commun. 2007 Aug 31;360(3):640-5. PMID:17612504 doi:10.1016/j.bbrc.2007.06.091
  2. ↑ Ito S, Taguchi H, Hamada S, Kawauchi S, Ito H, Senoura T, Watanabe J, Nishimukai M, Ito S, Matsui H. Enzymatic properties of cellobiose 2-epimerase from Ruminococcus albus and the synthesis of rare oligosaccharides by the enzyme. Appl Microbiol Biotechnol. 2008 Jun;79(3):433-41. PMID:18392616 doi:10.1007/s00253-008-1449-7
  3. ↑ Fujiwara T, Saburi W, Inoue S, Mori H, Matsui H, Tanaka I, Yao M. Crystal structure of Ruminococcus albus cellobiose 2-epimerase: structural insights into epimerization of unmodified sugar. FEBS Lett. 2013 Apr 2;587(7):840-6. doi: 10.1016/j.febslet.2013.02.007. Epub 2013, Feb 24. PMID:23462136 doi:10.1016/j.febslet.2013.02.007

3vw5, resolution 2.60Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3vw5&oldid=3941444"