3pzf

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1.75A resolution structure of Serpin-2 from Anopheles gambiae1.75A resolution structure of Serpin-2 from Anopheles gambiae

Structural highlights

3pzf is a 1 chain structure with sequence from Anopheles gambiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SP2_ANOGA Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine proteases CLIPB9 and CLIPB10, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656).[1] [2] [3] [4] [5] [6]

See Also

References

  1. Michel K, Budd A, Pinto S, Gibson TJ, Kafatos FC. Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei. EMBO Rep. 2005 Sep;6(9):891-7. PMID:16113656 doi:10.1038/sj.embor.7400478
  2. An C, Budd A, Kanost MR, Michel K. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-39. PMID:20953892 doi:10.1007/s00018-010-0543-z
  3. An C, Lovell S, Kanost MR, Battaile KP, Michel K. Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins. 2011 Feb 3. doi: 10.1002/prot.23002. PMID:21465556 doi:10.1002/prot.23002
  4. Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K. Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 from the Malaria Vector Anopheles gambiae. J Biol Chem. 2015 Jan 30;290(5):2946-56. doi: 10.1074/jbc.M114.625665. Epub 2014 , Dec 17. PMID:25525260 doi:http://dx.doi.org/10.1074/jbc.M114.625665
  5. Sousa GL, Bishnoi R, Baxter RHG, Povelones M. The CLIP-domain serine protease CLIPC9 regulates melanization downstream of SPCLIP1, CLIPA8, and CLIPA28 in the malaria vector Anopheles gambiae. PLoS Pathog. 2020 Oct 12;16(10):e1008985. PMID:33045027 doi:10.1371/journal.ppat.1008985
  6. Zhang X, Li M, El Moussawi L, Saab S, Zhang S, Osta MA, Michel K. CLIPB10 is a Terminal Protease in the Regulatory Network That Controls Melanization in the African Malaria Mosquito Anopheles gambiae. Front Cell Infect Microbiol. 2021 Jan 15;10:585986. PMID:33520733 doi:10.3389/fcimb.2020.585986

3pzf, resolution 1.75Å

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OCA