Crystal structure of PPO from bacillus subtilis with AFCrystal structure of PPO from bacillus subtilis with AF
Structural highlights
3i6d is a 2 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
CGOX_BACSU Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:7928957, PubMed:9217019). Catalyzes the oxidation of coproporphyrinogen III to coproporphyrin III (PubMed:8288631, PubMed:7928957, PubMed:9217019, PubMed:9784236). Can also oxidize protoporphyrinogen IX to protoporphyrin-IX (PubMed:8288631, PubMed:7928957, PubMed:9217019, PubMed:9784236, PubMed:19944166). The specific activity for the oxidation of coproporphyrinogen III is much higher than that for the oxidation of protoporphyrinogen IX (PubMed:7928957, PubMed:9217019). Can also oxidize mesoporphyrinogen IX, but not uroporphyrinogen III (PubMed:8288631, PubMed:9784236, PubMed:7928957).[1][2][3][4][5]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑Qin X, Sun L, Wen X, Yang X, Tan Y, Jin H, Cao Q, Zhou W, Xi Z, Shen Y. Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis. J Struct Biol. 2010 Apr;170(1):76-82. Epub 2009 Nov 26. PMID:19944166 doi:10.1016/j.jsb.2009.11.012
↑Hansson M, Hederstedt L. Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX. J Bacteriol. 1994 Oct;176(19):5962-70. PMID:7928957 doi:10.1128/jb.176.19.5962-5970.1994
↑Dailey TA, Meissner P, Dailey HA. Expression of a cloned protoporphyrinogen oxidase. J Biol Chem. 1994 Jan 14;269(2):813-5 PMID:8288631
↑Hansson M, Gustafsson MC, Kannangara CG, Hederstedt L. Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity. Biochim Biophys Acta. 1997 Jun 20;1340(1):97-104. PMID:9217019 doi:10.1016/s0167-4838(97)00030-7
↑Corrigall AV, Siziba KB, Maneli MH, Shephard EG, Ziman M, Dailey TA, Dailey HA, Kirsch RE, Meissner PN. Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis. Arch Biochem Biophys. 1998 Oct 15;358(2):251-6. PMID:9784236 doi:10.1006/abbi.1998.0834
