2n67

C-terminal domain of Hemolysin II-P87M-BMRBC-terminal domain of Hemolysin II-P87M-BMRB

Structural highlights 2n67 is a 1 chain structure with sequence from Bacillus cereus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q81AN8_BACCR Publication Abstract from PubMed In addition to multiple virulence factors, Bacillus cereus a pathogen that causes food poisoning and life-threatening wound infections, secretes the pore-forming toxin hemolysin II (HlyII). The HlyII toxin has a unique 94 amino acid C-terminal domain (HlyIIC). HlyIIC exhibits splitting of NMR resonances due to cis/trans isomerization of a single proline near the C-terminus. To overcome heterogeneity, we solved the structure of P405M-HlyIIC, a mutant that exclusively stabilizes the trans state. The NMR structure of HlyIIC reveals a novel fold, consisting of two subdomains alphaA-beta1-beta2 and beta3-beta4-alphaB-beta5, that come together in a barrel-like structure. The barrel core is fastened by three layers of hydrophobic residues. The barrel end opposite the HlyIIC-core has a positively charged surface, that by binding negatively charged moieties on cellular membranes, may play a role in target-cell surface recognition or stabilization of the heptameric pore complex. In the WT domain, dynamic flexibility occurs at the N-terminus and the first alpha-helix that connects the HlyIIC domain to the HlyII-core structure. In the destabilizing P405M mutant, increased flexibility is evident throughout the first subdomain, suggesting that the HlyIIC structure may have arisen through gene fusion. NMR structure of the Bacillus cereus hemolysin II C-terminal domain reveals a novel fold.,Kaplan AR, Kaus K, De S, Olson R, Alexandrescu AT Sci Rep. 2017 Jun 12;7(1):3277. doi: 10.1038/s41598-017-02917-4. PMID:28607368 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hemolysin 3D structures

spinqualitylabelsall models 2n67 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image