2lum

Three-State Ensemble obtained from eNOEs of the Third Immunoglobulin Binding Domain of Protein G (GB3)Three-State Ensemble obtained from eNOEs of the Third Immunoglobulin Binding Domain of Protein G (GB3)

Structural highlights 2lum is a 1 chain structure with sequence from Streptococcus sp. 'group G'. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SPG2_STRSG Publication Abstract from PubMed Proteins are inherently dynamic systems whose motions cover large ranges in both magnitude and timescale. Because of the omnipresence of motion, it is likely that dynamics have important roles in the function of biomolecules. For detailed understanding of a protein's function, the three-dimensional structure and description of its dynamics are therefore required. Structure determination methods are well established, and NMR-relaxation phenomena provide insights into local molecular dynamics; moreover, recently several attempts have been made to detect concerted motion. Here, we present an ensemble-based structure-determination protocol using ensemble-averaged distance restraints obtained from exact NOE rates. Application to the model protein GB3 establishes an ensemble of structures that reveals correlated motion across the beta-sheet, concerted motion between the backbone and side chains localized in the structure core, and a lack of concerted conformational exchange between the beta-sheet and the alpha-helix. Spatial elucidation of motion in proteins by ensemble-based structure calculation using exact NOEs.,Vogeli B, Kazemi S, Guntert P, Riek R Nat Struct Mol Biol. 2012 Sep 2. doi: 10.1038/nsmb.2355. PMID:22940676 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 2lum Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image