2ljh

From Proteopedia
Jump to navigation Jump to search

NMR structure of Double-stranded RNA-specific editase AdarNMR structure of Double-stranded RNA-specific editase Adar

Structural highlights

2ljh is a 1 chain structure with sequence from Drosophila melanogaster. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ADAR_DROME Has A-to-I RNA editing activity on extended dsRNA: edits RNA-binding protein Rnp4F. A-to-I editing of pre-mRNAs acts predominantly through nervous system targets to affect adult nervous system integrity, function and behavior. Essential for adaptation to environmental stresses, such as oxygen deprivation, and for the prevention of premature neuronal degeneration, through the editing of ion channels as targets.[1] [2] [3] [4]

Publication Abstract from PubMed

Adenosine deaminases that act on RNA (ADAR) catalyze adenosine to inosine (A-to-I) editing in double-stranded RNA (dsRNA) substrates. Inosine is read as guanosine by the translation machinery; therefore A-to-I editing events in coding sequences may result in recoding genetic information. Whereas vertebrates have two catalytically active enzymes, namely ADAR1 and ADAR2, Drosophila has a single ADAR protein (dADAR) related to ADAR2. The structural determinants controlling substrate recognition and editing of a specific adenosine within dsRNA substrates are only partially understood. Here, we report the solution structure of the N-terminal dsRNA binding domain (dsRBD) of dADAR and use NMR chemical shift perturbations to identify the protein surface involved in RNA binding. Additionally, we show that Drosophila ADAR edits the R/G site in the mammalian GluR-2 pre-mRNA which is naturally modified by both ADAR1 and ADAR2. We then constructed a model showing how dADAR dsRBD1 binds to the GluR-2 R/G stem-loop. This model revealed that most side chains interacting with the RNA sugar-phosphate backbone need only small displacement to adapt for dsRNA binding and are thus ready to bind to their dsRNA target. It also predicts that dADAR dsRBD1 would bind to dsRNA with less sequence specificity than dsRBDs of ADAR2. Altogether, this study gives new insights into dsRNA substrate recognition by Drosophila ADAR.

Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA.,Barraud P, Heale BS, O'Connell MA, Allain FH Biochimie. 2011 Dec 23. PMID:22210494[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Palladino MJ, Keegan LP, O'Connell MA, Reenan RA. dADAR, a Drosophila double-stranded RNA-specific adenosine deaminase is highly developmentally regulated and is itself a target for RNA editing. RNA. 2000 Jul;6(7):1004-18. PMID:10917596
  2. ↑ Ma E, Gu XQ, Wu X, Xu T, Haddad GG. Mutation in pre-mRNA adenosine deaminase markedly attenuates neuronal tolerance to O2 deprivation in Drosophila melanogaster. J Clin Invest. 2001 Mar;107(6):685-93. PMID:11254668 doi:http://dx.doi.org/10.1172/JCI11625
  3. ↑ Palladino MJ, Keegan LP, O'Connell MA, Reenan RA. A-to-I pre-mRNA editing in Drosophila is primarily involved in adult nervous system function and integrity. Cell. 2000 Aug 18;102(4):437-49. PMID:10966106
  4. ↑ Peters NT, Rohrbach JA, Zalewski BA, Byrkett CM, Vaughn JC. RNA editing and regulation of Drosophila 4f-rnp expression by sas-10 antisense readthrough mRNA transcripts. RNA. 2003 Jun;9(6):698-710. PMID:12756328
  5. ↑ Barraud P, Heale BS, O'Connell MA, Allain FH. Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA. Biochimie. 2011 Dec 23. PMID:22210494 doi:10.1016/j.biochi.2011.12.017
Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=2ljh&oldid=4152327"