E. coli FtsK gamma domainE. coli FtsK gamma domain
Structural highlights
2j5p is a 1 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
FTSK_ECOLI Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif. Stoppage of translocation is accompanied by a reduction in ATPase activity. Also stimulates topoisomerase 4 activity. Required for the targeting of FtsQ, FtsL and FtsI to the septum.[1][2][3][4][5][6][7][8][9][10][11]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The bacterial septum-located DNA translocase FtsK coordinates circular chromosome segregation with cell division. Rapid translocation of DNA by FtsK is directed by 8-base-pair DNA motifs (KOPS), so that newly replicated termini are brought together at the developing septum, thereby facilitating completion of chromosome segregation. Translocase functions reside in three domains, alpha, beta and gamma. FtsKalphabeta are necessary and sufficient for ATP hydrolysis-dependent DNA translocation, which is modulated by FtsKgamma through its interaction with KOPS. By solving the FtsKgamma structure by NMR, we show that gamma is a winged-helix domain. NMR chemical shift mapping localizes the DNA-binding site on the gamma domain. Mutated proteins with substitutions in the FtsKgamma DNA-recognition helix are impaired in DNA binding and KOPS recognition, yet remain competent in DNA translocation and XerCD-dif site-specific recombination, which facilitates the late stages of chromosome segregation.
The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS.,Sivanathan V, Allen MD, de Bekker C, Baker R, Arciszewska LK, Freund SM, Bycroft M, Lowe J, Sherratt DJ Nat Struct Mol Biol. 2006 Nov;13(11):965-72. Epub 2006 Oct 22. PMID:17057717[12]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑Yu XC, Weihe EK, Margolin W. Role of the C terminus of FtsK in Escherichia coli chromosome segregation. J Bacteriol. 1998 Dec;180(23):6424-8. PMID:9829960
↑Wang L, Lutkenhaus J. FtsK is an essential cell division protein that is localized to the septum and induced as part of the SOS response. Mol Microbiol. 1998 Aug;29(3):731-40. PMID:9723913
↑Chen JC, Beckwith J. FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division. Mol Microbiol. 2001 Oct;42(2):395-413. PMID:11703663
↑Aussel L, Barre FX, Aroyo M, Stasiak A, Stasiak AZ, Sherratt D. FtsK Is a DNA motor protein that activates chromosome dimer resolution by switching the catalytic state of the XerC and XerD recombinases. Cell. 2002 Jan 25;108(2):195-205. PMID:11832210
↑Bigot S, Corre J, Louarn JM, Cornet F, Barre FX. FtsK activities in Xer recombination, DNA mobilization and cell division involve overlapping and separate domains of the protein. Mol Microbiol. 2004 Nov;54(4):876-86. PMID:15522074 doi:http://dx.doi.org/10.1111/j.1365-2958.2004.04335.x
↑Yates J, Zhekov I, Baker R, Eklund B, Sherratt DJ, Arciszewska LK. Dissection of a functional interaction between the DNA translocase, FtsK, and the XerD recombinase. Mol Microbiol. 2006 Mar;59(6):1754-66. PMID:16553881 doi:http://dx.doi.org/10.1111/j.1365-2958.2005.05033.x
↑Kennedy SP, Chevalier F, Barre FX. Delayed activation of Xer recombination at dif by FtsK during septum assembly in Escherichia coli. Mol Microbiol. 2008 May;68(4):1018-28. doi: 10.1111/j.1365-2958.2008.06212.x., Epub 2008 Mar 19. PMID:18363794 doi:http://dx.doi.org/10.1111/j.1365-2958.2008.06212.x
↑Graham JE, Sivanathan V, Sherratt DJ, Arciszewska LK. FtsK translocation on DNA stops at XerCD-dif. Nucleic Acids Res. 2010 Jan;38(1):72-81. doi: 10.1093/nar/gkp843. Epub 2009 Oct, 23. PMID:19854947 doi:http://dx.doi.org/10.1093/nar/gkp843
↑Bigot S, Marians KJ. DNA chirality-dependent stimulation of topoisomerase IV activity by the C-terminal AAA+ domain of FtsK. Nucleic Acids Res. 2010 May;38(9):3031-40. doi: 10.1093/nar/gkp1243. Epub 2010, Jan 16. PMID:20081205 doi:http://dx.doi.org/10.1093/nar/gkp1243
↑Grainge I, Lesterlin C, Sherratt DJ. Activation of XerCD-dif recombination by the FtsK DNA translocase. Nucleic Acids Res. 2011 Jul;39(12):5140-8. doi: 10.1093/nar/gkr078. Epub 2011 Mar, 2. PMID:21371996 doi:http://dx.doi.org/10.1093/nar/gkr078
↑Sivanathan V, Allen MD, de Bekker C, Baker R, Arciszewska LK, Freund SM, Bycroft M, Lowe J, Sherratt DJ. The FtsK gamma domain directs oriented DNA translocation by interacting with KOPS. Nat Struct Mol Biol. 2006 Nov;13(11):965-72. Epub 2006 Oct 22. PMID:17057717 doi:10.1038/nsmb1158
