2e0c
crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain7 at 2.0 A resolutioncrystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain7 at 2.0 A resolution
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedIsocitrate dehydrogenase is a catabolic enzyme that acts during the third step of the tricarboxylic acid cycle. The hypothetical protein ST2166 from the archaeon Sulfolobus tokodaii was isolated and crystallized. It shares high primary structure homology with prokaryotic NADP(+)-dependent IDHs, suggesting that these enzymes share a common enzymatic mechanism. The crystal structure of ST2166 was determined at 2.0 A resolution in the apo form, and then the structure of the crystal soaked with NADP(+) was also determined at 2.4 A resolution, which contained NADP(+) bound at the putative active site. Comparisons between the structures of apo and NADP(+)-bound forms and NADP-IDHs from other prokaryotes suggest that prokaryotic NADP-IDHs recognize their cofactors using conserved Lys335, Tyr336, and Arg386 in ST2166 at the opening cleft before the domain closure. Crystal Structures of the Putative Isocitrate Dehydrogenase from Sulfolobus tokodaii Strain 7 in the Apo and NADP(+)-Bound Forms.,Kondo H, Murakami M Archaea. 2018 Dec 19;2018:7571984. doi: 10.1155/2018/7571984. eCollection 2018. PMID:30662370[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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