Structural Basis for UTP Specificity of RNA Editing TUTases from Trypanosoma BruceiStructural Basis for UTP Specificity of RNA Editing TUTases from Trypanosoma Brucei
Structural highlights
2b51 is a 1 chain structure with sequence from Trypanosoma brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
TUT2_TRYBB Terminal uridylyltransferase which, as part of the mitochondrial RNA editing core complex (RECC), is involved in the post-transcriptional editing of mitochondrial RNA, a process involving the addition and deletion of uridine (U) nucleotides in the pre-mRNA (PubMed:12820966, PubMed:19465686, PubMed:20362585). Specifically, catalyzes the addition of one U to single-stranded RNA with a preference for a 3'-terminal A or G and adds the number of Us specified by a guide RNA (gRNA) to precleaved double-stranded RNA editing substrates (PubMed:12820966, PubMed:19465686, PubMed:20362585, PubMed:16281058). Essential for the survival of the bloodstream form (PubMed:16281058).[1][2][3][4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑Ernst NL, Panicucci B, Igo RP Jr, Panigrahi AK, Salavati R, Stuart K. TbMP57 is a 3' terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome. Mol Cell. 2003 Jun;11(6):1525-36. PMID:12820966 doi:10.1016/s1097-2765(03)00185-0
↑Deng J, Ernst NL, Turley S, Stuart KD, Hol WG. Structural basis for UTP specificity of RNA editing TUTases from Trypanosoma brucei. EMBO J. 2005 Dec 7;24(23):4007-17. Epub 2005 Nov 10. PMID:16281058
↑Aphasizheva I, Ringpis GE, Weng J, Gershon PD, Lathrop RH, Aphasizhev R. Novel TUTase associates with an editosome-like complex in mitochondria of Trypanosoma brucei. RNA. 2009 Jul;15(7):1322-37. PMID:19465686 doi:10.1261/rna.1538809
↑Ringpis GE, Aphasizheva I, Wang X, Huang L, Lathrop RH, Hatfield GW, Aphasizhev R. Mechanism of U insertion RNA editing in trypanosome mitochondria: the bimodal TUTase activity of the core complex. J Mol Biol. 2010 Jun 25;399(5):680-95. PMID:20362585 doi:10.1016/j.jmb.2010.03.050
