Structure of the Group II Intron Splicing Factor CRS2Structure of the Group II Intron Splicing Factor CRS2
Structural highlights
1rym is a 1 chain structure with sequence from Zea mays. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
CRS2_MAIZE Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity.[1][2][3][4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑Jenkins BD, Barkan A. Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts. EMBO J. 2001 Feb 15;20(4):872-9. PMID:11179231 doi:http://dx.doi.org/10.1093/emboj/20.4.872
↑Jenkins BD, Kulhanek DJ, Barkan A. Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors. Plant Cell. 1997 Mar;9(3):283-96. PMID:9090875 doi:http://dx.doi.org/10.1105/tpc.9.3.283
↑Vogel J, Borner T, Hess WR. Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants. Nucleic Acids Res. 1999 Oct 1;27(19):3866-74. PMID:10481026
↑Ostheimer GJ, Williams-Carrier R, Belcher S, Osborne E, Gierke J, Barkan A. Group II intron splicing factors derived by diversification of an ancient RNA-binding domain. EMBO J. 2003 Aug 1;22(15):3919-29. PMID:12881426 doi:http://dx.doi.org/10.1093/emboj/cdg372
