1o6v
Internalin (INLA, Listeria monocytogenes) - functional domain, uncomplexedInternalin (INLA, Listeria monocytogenes) - functional domain, uncomplexed
Structural highlights
FunctionINLA_LISMO Mediates the entry of L.monocytogenes into cells. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedListeria monocytogenes, a food-borne bacterial pathogen, enters mammalian cells by inducing its own phagocytosis. The listerial protein internalin (InlA) mediates bacterial adhesion and invasion of epithelial cells in the human intestine through specific interaction with its host cell receptor E-cadherin. We present the crystal structures of the functional domain of InlA alone and in a complex with the extracellular, N-terminal domain of human E-cadherin (hEC1). The leucine rich repeat (LRR) domain of InlA surrounds and specifically recognizes hEC1. Individual interactions were probed by mutagenesis and analytical ultracentrifugation. These include Pro16 of hEC1, a major determinant for human susceptibility to L. monocytogenes infection that is essential for intermolecular recognition. Our studies reveal the structural basis for host tro-pism of this bacterium and the molecular deception L. monocytogenes employs to exploit the E-cadherin system. Structure of internalin, a major invasion protein of Listeria monocytogenes, in complex with its human receptor E-cadherin.,Schubert WD, Urbanke C, Ziehm T, Beier V, Machner MP, Domann E, Wehland J, Chakraborty T, Heinz DW Cell. 2002 Dec 13;111(6):825-36. PMID:12526809[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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