1ku4

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

STRUCTURE MODEL OF THE SMUC159 ANTIBODY SINGLE CHAIN FRAGMENT SPECIFIC FOR MUCIN-1STRUCTURE MODEL OF THE SMUC159 ANTIBODY SINGLE CHAIN FRAGMENT SPECIFIC FOR MUCIN-1

Structural highlights For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, PDBsum, ProSAT Publication Abstract from PubMed Mucin-1 has proven to be a suitable target for antibody-based diagnosis and therapy of certain tumours, but no appropriate human antibody or antibody fragment displaying slow dissociation rate kinetics against this target is available. Since a rapid dissociation character prevents an antibody fragment from remaining at the site of the antigen, this fact may prevent the successful application of a human mucin-1 specific antibody in diagnosis and therapy. We have now used iterative antibody libraries to evolve a human antibody fragment originally obtained from a naive antibody library. A strategy was devised whereby molecular variants displaying slow dissociation kinetics against the repetitive mucin-1 tumour-associated antigen can be selected in vitro. The evolved clones, that allowed for a reduced dissociation from the tumour antigen, carried substitutions in the outer parts of the binding site. This demonstrated the ability of this in vitro evolution technique to mimic the process whereby antibodies evolve in vivo. We have thus devised a strategy through which molecular variants displaying slow dissociation from a repetitive target like the mucin-1 tumour-associated antigen can be obtained in vitro. These or related molecules obtained by this approach will serve as a starting point for the development of fully human antibodies for use in mucin-1 specific tumour therapy of diagnosis. Antibody evolution from the centre to the periphery: applied to a human antibody fragment recognising the tumour-associated antigen mucin-1.,Jirholt P, Borrebaeck CA, Ohlin M J Mol Biol. 2002 Apr 26;318(2):407-16. PMID:12051847 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 1ku4 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image