Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7Crystal Structure of gp2.5, a Single-Stranded DNA Binding Protein Encoded by Bacteriophage T7
Structural highlights
1je5 is a 2 chain structure with sequence from Escherichia phage T7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
SSB_BPT7 Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks (PubMed:16807232, PubMed:8617248, PubMed:9079662, PubMed:11222583). Coats the lagging-strand ssDNA as the replication fork advances and stimulates the activities of viral DNA polymerase and primase/helicase (PubMed:8617248). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:9651583). Together with DNA primase/helicase, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:8617248). Promotes also the formation of joint molecules (PubMed:8617248, PubMed:9079662). Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation (PubMed:1634538).[HAMAP-Rule:MF_04153][1][2][3][4][5][6]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑Yu M, Masker W. T7 single strand DNA binding protein but not T7 helicase is required for DNA double strand break repair. J Bacteriol. 2001 Mar;183(6):1862-9. PMID:11222583 doi:10.1128/JB.183.6.1862-1869.2001
↑Kim YT, Tabor S, Bortner C, Griffith JD, Richardson CC. Purification and characterization of the bacteriophage T7 gene 2.5 protein. A single-stranded DNA-binding protein. J Biol Chem. 1992 Jul 25;267(21):15022-31. PMID:1634538
↑Marintcheva B, Hamdan SM, Lee SJ, Richardson CC. Essential residues in the C terminus of the bacteriophage T7 gene 2.5 single-stranded DNA-binding protein. J Biol Chem. 2006 Sep 1;281(35):25831-40. Epub 2006 Jun 28. PMID:16807232 doi:http://dx.doi.org/10.1074/jbc.M604601200
↑Kong D, Richardson CC. Single-stranded DNA binding protein and DNA helicase of bacteriophage T7 mediate homologous DNA strand exchange. EMBO J. 1996 Apr 15;15(8):2010-9 PMID:8617248
↑Kong D, Nossal NG, Richardson CC. Role of the bacteriophage T7 and T4 single-stranded DNA-binding proteins in the formation of joint molecules and DNA helicase-catalyzed polar branch migration. J Biol Chem. 1997 Mar 28;272(13):8380-7. PMID:9079662 doi:10.1074/jbc.272.13.8380
↑Lee J, Chastain PD 2nd, Kusakabe T, Griffith JD, Richardson CC. Coordinated leading and lagging strand DNA synthesis on a minicircular template. Mol Cell. 1998 Jun;1(7):1001-10. PMID:9651583 doi:10.1016/s1097-2765(00)80100-8
