1g2r
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Structure of Cytosolic Protein of Unknown Function Coded by Gene from NUSA/INFB Region, a YlxR Homologue
OverviewOverview
The structure of the YlxR protein of unknown function from Streptococcus, pneumonia was determined to 1.35 A. YlxR is expressed from the nusA/infB, operon in bacteria and belongs to a small protein family (COG2740) that, shares a conserved sequence motif GRGA(Y/W). The family shows no, significant amino-acid sequence similarity with other proteins., Three-wavelength diffraction MAD data were collected to 1.7 A from, orthorhombic crystals using synchrotron radiation and the structure was, determined using a semi-automated approach. The YlxR structure resembles a, two-layer alpha/beta sandwich with the overall shape of a cylinder and, shows no structural homology to proteins of known structure. Structural, analysis revealed that the YlxR structure represents a new protein fold, that belongs to the alpha-beta plait superfamily. The distribution of the, electrostatic surface potential shows a large positively charged patch on, one side of the protein, a feature often found in nucleic acid-binding, proteins. Three sulfate ions bind to this positively charged surface., Analysis of potential binding sites uncovered several substantial clefts, with the largest spanning 3/4 of the protein. A similar distribution of, binding sites and a large sharply bent cleft are observed in RNA-binding, proteins that are unrelated in sequence and structure. It is proposed that, YlxR is an RNA-binding protein.
About this StructureAbout this Structure
1G2R is a Single protein structure of sequence from Streptococcus pneumoniae with SO4 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Streptococcus pneumonia YlxR at 1.35 A shows a putative new fold., Osipiuk J, Gornicki P, Maj L, Dementieva I, Laskowski R, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2001 Nov;57(Pt 11):1747-51. Epub 2001, Oct 25. PMID:11679764
Page seeded by OCA on Sun Nov 25 00:44:48 2007