User:Tommie Hata/Introduction to Protein Engineering-Subtilisin
This Protein Engineering module has been developed using material from Dr. Scott Banta's course, Protein Engineering (chemical engineering department at Columbia University).
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The structure to the right is subtilisin BPN' and streptomyces subtilisin inhibitor (PDB ID: 2sic). The subtilisin enzyme is colored white while the Streptomyces subtilisin inhibitor is colored yellow. By , we can take a look at how the substrate binds the active site. Enzyme-inhibitor structures are more offen solved and deposited in the Protein Data Bank (PDB)compared to enzyme-substrate structures. This is because enzyme-substrate complexes are often transient by nature. Without being able to form a stable enzyme-substrate complex, crystals of the complex cannot be grown so that the structure can be solved through x-ray crystallography.
, which has been minimized in the view to highlight the chain bound in the active site of subtilisin.