1sf0
BACKBONE SOLUTION STRUCTURE OF MIXED ALPHA/BETA PROTEIN PF1061
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OverviewOverview
Structural genomics (or proteomics) activities are critically dependent on, the availability of high-throughput structure determination methodology., Development of such methodology has been a particular challenge for NMR, based structure determination because of the demands for isotopic labeling, of proteins and the requirements for very long data acquisition times. We, present here a methodology that gains efficiency from a focus on, determination of backbone structures of proteins as opposed to full, structures with all sidechains in place. This focus is appropriate given, the presumption that many protein structures in the future will be built, using computational methods that start from representative fold family, structures and replace as many as 70% of the sidechains in the course of, structure determination. The methodology we present is based primarily on, residual dipolar couplings (RDCs), readily accessible NMR observables that, constrain the orientation of backbone fragments irrespective of separation, in space. A new software tool is described for the assembly of backbone, fragments under RDC constraints and an application to a structural, genomics target is presented. The target is an 8.7 kDa protein from, Pyrococcus furiosus, PF1061, that was previously not well annotated, and, had a nearest structurally characterized neighbor with only 33% sequence, identity. The structure produced shows structural similarity to this, sequence homologue, but also shows similarity to other proteins, which, suggests a functional role in sulfur transfer. Given the backbone, structure and a possible functional link this should be an ideal target, for development of modeling methods.
About this StructureAbout this Structure
1SF0 is a Single protein structure of sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
ReferenceReference
Backbone solution structures of proteins using residual dipolar couplings: application to a novel structural genomics target., Valafar H, Mayer KL, Bougault CM, LeBlond PD, Jenney FE Jr, Brereton PS, Adams MW, Prestegard JH, J Struct Funct Genomics. 2004;5(4):241-54. PMID:15704012
Page seeded by OCA on Sun Nov 25 00:22:36 2007