1wl1

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Revision as of 01:01, 25 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1wl1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wl1, resolution 3.45Å" /> '''Crystal Structure Of...)
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File:1wl1.gif


1wl1, resolution 3.45Å

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Crystal Structure Of Octaprenyl Pyrophosphate Synthase From Hyperthermophilic Thermotoga Maritima H74A mutant

OverviewOverview

Octaprenyl pyrophosphate synthase (OPPs) catalyzes the condensation of, five isopentenyl pyrophosphates with farnesyl pyrophosphate to generate, C(40) octaprenyl pyrophosphate. The enzymes from the hyperthermophilic, bacterium Thermotoga maritima and from the mesophilic Escherichia coli, were expressed in E. coli and the recombinant proteins were purified and, crystallized. The T. maritima OPPs crystals belong to space group P42(1)2, with unit-cell parameters a = b = 151.53, c = 69.72 A. The E. coli OPPs, crystals belong to space group C222(1), with unit-cell parameters a =, 247.66, b = 266.10, c = 157.93 A. Diffraction data were collected at 100 K, using synchrotron radiation and an in-house X-ray source. Structure, determination of T. maritima OPPs has been carried out using MIR data sets, at 2.8 A resolution. The asymmetric unit contains one dimer. An initial, model with 280 residues per subunit has been built and refined to 2.28 A, resolution. It shows mostly helical structure and resembles that of avian, farnesyl pyrophosphate synthase.

About this StructureAbout this Structure

1WL1 is a Single protein structure of sequence from Thermotoga maritima msb8. Active as Trans-octaprenyltranstransferase, with EC number 2.5.1.11 Full crystallographic information is available from OCA.

ReferenceReference

Preliminary X-ray diffraction analysis of octaprenyl pyrophosphate synthase crystals from Thermotoga maritima and Escherichia coli., Guo RT, Ko TP, Chou CC, Shr HL, Chu HM, Tsai YH, Liang PH, Wang AH, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2265-8. Epub 2003, Nov 27. PMID:14646090

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