1wcj
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NMR structure of a proteins with unknown function from DUF59 family (TM0487) from Thermotoga maritima.
OverviewOverview
The NMR structure of the conserved hypothetical protein TM0487 from, Thermotoga maritima represents an alpha/beta-topology formed by the, regular secondary structures alpha1-beta1-beta2-alpha2-beta3-beta4-alpha3-, beta5-3(10)-alpha4, with a small anti-parallel beta-sheet of beta-strands, 1 and 2, and a mixed parallel/anti-parallel beta-sheet of beta-strands, 3-5. Similar folds have previously been observed in other proteins, with, amino acid sequence identity as low as 3% and a variety of different, functions. There are also 216 sequence homologs of TM0487, which all have, the signature sequence of domains of unknown function 59 (DUF59), for, which no three-dimensional structures have as yet been reported. The, TM0487 structure thus presents a platform for homology modeling of this, large group of DUF59 proteins. Conserved among most of the DUF59s are 13, hydrophobic residues, which are clustered in the core of TM0487. A, putative active site of TM0487 consisting of residues D20, E22, L23, T51, T52, and C55 is conserved in 98 of the 216 DUF59 sequences. Asp20 is, buried within the proposed active site without any compensating positive, charge, which suggests that its pK(a) value may be perturbed. Furthermore, the DUF59 family includes ORFs that are part of a conserved chromosomal, group of proteins predicted to be involved in Fe-S cluster metabolism.
About this StructureAbout this Structure
1WCJ is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure of the conserved hypothetical protein TM0487 from Thermotoga maritima: implications for 216 homologous DUF59 proteins., Almeida MS, Herrmann T, Peti W, Wilson IA, Wuthrich K, Protein Sci. 2005 Nov;14(11):2880-6. Epub 2005 Sep 30. PMID:16199668
Page seeded by OCA on Sat Nov 24 23:43:29 2007
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- Single protein
- Thermotoga maritima
- Almeida, M.S.
- Herrmann, T.
- JCSG, Joint.Center.for.Structural.Genomics.
- Peti, W.
- Wuthrich, K.
- Alpha/beta fold
- Contains paad domain
- Hypothetical protein
- Jcsg
- Joint center for structural genomics
- Protein structure initiative
- Psi
- Similar to paad protein
- Structural genomics
- Unknown activity