1nb2
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Crystal Structure of Nucleoside Diphosphate Kinase from Bacillus Halodenitrificans
OverviewOverview
We found that when grown under anaerobic conditions the moderate, halophile, gram-positive bacterium Bacillus halodenitrificans (ATCC 49067), synthesizes large amounts of a polypeptide complex that contains a heme, center capable of reversibly bind nitric oxide. This complex, when exposed, to air, dissociates and reassociates into two active components, a, Mn-containing superoxide dismutase (SOD) and a nucleoside diphosphate, kinase (BhNDK). The crystal structure of this latter enzyme has been, determined at 2.2A resolution using molecular replacement method, based on, the crystal structure of Drosophila melanogaster NDK. The model contains, 149 residues of a total 150 residues and 34 water molecules. BhNDK, consists of a four-stranded antiparallel beta-sheet, whose surfaces are, partially covered by six alpha-helices, and its overall and active site, structures are similar to those of homologous enzymes. However, the, hexameric packing of BhNDK shows that this enzyme is different from both, eukaryotic and gram-negative bacteria. The need for the bacterium to, presynthesize both SOD and NDK precursors which are activated during the, anaerobic-aerobic transition is discussed.
About this StructureAbout this Structure
1NB2 is a Single protein structure of sequence from Virgibacillus halodenitrificans. Active as Nucleoside-diphosphate kinase, with EC number 2.7.4.6 Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a nucleoside diphosphate kinase from Bacillus halodenitrificans: coexpression of its activity with a Mn-superoxide dismutase., Chen CJ, Liu MY, Chang T, Chang WC, Wang BC, Le Gall J, J Struct Biol. 2003 May;142(2):247-55. PMID:12713952
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