197d

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Revision as of 23:06, 24 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="197d" size="450" color="white" frame="true" align="right" spinBox="true" caption="197d, resolution 2.190Å" /> '''ORTHORHOMBIC CRYSTA...)
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File:197d.gif


197d, resolution 2.190Å

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ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE

OverviewOverview

The X-ray crystal structure of the double-helical A-DNA octanucleotide, d(GTACGTAC) has been solved by molecular replacement and refined to a, resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516, observed reflections with F > 4 sigma(F). The sequence crystallizes as an, A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with, one duplex molecule solvated by 66 water molecules in the asymmetric unit., Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the, first time that such a crystal form has been observed. This orthorhombic, structure has been compared with the tetragonal structure of the same, oligonucleotide. It adopts a bent structure with an unusual packing, between symmetry-related molecules.

About this StructureAbout this Structure

197D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:8477741

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