1ec5
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CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL
OverviewOverview
De novo protein design provides an attractive approach for the, construction of models to probe the features required for function of, complex metalloproteins. The metal-binding sites of many metalloproteins, lie between multiple elements of secondary structure, inviting a, retrostructural approach to constructing minimal models of their active, sites. The backbone geometries comprising the metal-binding sites of zinc, fingers, diiron proteins, and rubredoxins may be described to within, approximately 1 A rms deviation by using a simple geometric model with, only six adjustable parameters. These geometric models provide excellent, starting points for the design of metalloproteins, as illustrated in the, construction of Due Ferro 1 (DF1), a minimal model for the Glu-Xxx-Xxx-His, class of dinuclear metalloproteins. This protein was synthesized and, structurally characterized as the di-Zn(II) complex by x-ray, crystallography, by using data that extend to 2.5 A. This four-helix, bundle protein is comprised of two noncovalently associated, helix-loop-helix motifs. The dinuclear center is formed by two bridging, Glu and two chelating Glu side chains, as well as two monodentate His, ligands. The primary ligands are mostly buried in the protein interior, and their geometries are stabilized by a network of hydrogen bonds to, second-shell ligands. In particular, a Tyr residue forms a hydrogen bond, to a chelating Glu ligand, similar to a motif found in the, diiron-containing R2 subunit of Escherichia coli ribonucleotide reductase, and the ferritins. DF1 also binds cobalt and iron ions and should provide, an attractive model for a variety of diiron proteins that use oxygen for, processes including iron storage, radical formation, and hydrocarbon, oxidation.
About this StructureAbout this Structure
1EC5 is a Protein complex structure of sequences from [1] with ZN, ACE and NH2 as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Inaugural article: retrostructural analysis of metalloproteins: application to the design of a minimal model for diiron proteins., Lombardi A, Summa CM, Geremia S, Randaccio L, Pavone V, DeGrado WF, Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6298-305. PMID:10841536
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